Prof. Lewis and his team are pioneering the study of mechanical-biological interactions in bone through innovative intravital imaging techniques. Their research focuses on osteocyte mechanotransduction and mechanobiology in vivo, utilizing fluorescent signals to observe cellular and molecular events in real-time. These novel approaches preserve critical endocrine and tissue crosstalk pathways, offering unprecedented insights into cellular behavior in both healthy and diseased states. In this presentation, Prof. Lewis will introduce cutting-edge methodologies and reveal recent findings on osteocyte mechanoregulation and endocytosis, advancing our understanding of bone biology and potential therapeutic interventions.